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This study was performed to determine the optimal dose of ozone for wound healing. Twenty Korean black goats were allocated to 4 groups. The skin wound with 1×1 cm was induced over the hoof. For 5 minutes per day, 0.1 ppm (Group Ⅰ), 1 ppm (Group Ⅱ) and 6 ppm (Group Ⅲ) ozone gas was applied. In control group, 5 ml of normal saline was applied daily on the wound. Wound square was determined using color image analyzer at day 0, 4 and 14. Soft tissue depth of wound was determined using radiograph. Before the application of ozone gas, 1×1 cm of skin tissue was resected and observed for histopathologic findings. To determine the healing effect 2×1 cm of wound tissue was resected and observed for histopathologic findings at day 14. In mean squares of wound, all experimental groups revealed more reduced mean squares than control group at day 4, and especially Group Ⅱ (p<0.05) and Group Ⅲ (p<0.05) revealed significant reduction. At day 14, Group Ⅰ and Group Ⅱ (p<0.05) revealed more reduced mean squares than control group, but Group Ⅲ revealed less reduced mean squares than control group. In soft tissue depth of wound, all experimental groups and control group revealed swelling at day 4, compared with day 1. At day 14, all experimental groups and control group revealed reduction, compared with day 4, and especially Group Ⅱ (p<0.05) revealed significant reduction. In histopathological findings, inflammation findings in epidermis and dermis, hemorrhagic funding in epidermis were revealed in control group. In Group Ⅰ, inflammation finding was reduced, compared with control group. Formation of scab and many reepithelialization of epidermis were revealed. In Group Ⅱ, inflammation findings in epidermis and dermis which were observed in control group were not found, normal skin tissue was revealed. In Group Ⅲ, inflammation findings in epidermis and dermis were revealed, reepithlialization of epidermis was not founded. Slow healing process was observed, compared with Group Ⅰ and Ⅲ. It was concluded that Group Ⅱ has excellent promotion effect of wound healing.
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